Abstract:

DNA fingerprinting is a relatively new technique by which a set of polymorphic markers can be simultaneously detected, resulting in a pattern unique to a species, strain or individual (DNA fingerprint). The DNA fingerprinting probes applicable to fish include human minisatellite DNAs, bacteriophage Ml3, mouse sequence related to Drosophila Per gene and some oligonucleotide sequences such as (GATA)4 and (GACA)4. the fingerprints can also be obtained by combination of a number of hypervariable single locus markers. The methods employed to reveal DNA fingerprints include hybridization and the polymerase chain reaction (PCR). With arbitrarily-primed PCR, DNA fingerprints can be obtained from a wide range of species. The technique has proven to be a powerful tool in various fields of fish biology, such as individual and stock identification and breeding.

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Date 1993/10/01

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